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An experiment was conducted in which his-trp-leu+ mutants were crossed with his+trp+ and leu- mutant

An experiment was conducted in which his-trp-leu+ mutants were crossed with his+trp+ and leu- mutants.However, no recombinants occurred when a filter was placed in the U-shaped Davis tube to separate the strains.  Why was that?a.    they were only plated on minimal media, instead of on nutrient agar firstb.    they needed conjugation to occurc.    they needed transduction to occurd.    they needed transformation to occure.    they needed lygogeneis to occur

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