You are studying genes and DNAsequences involved in the regulation of the grandoperon.When your lab’s strain of e. coli are in the presence of the sugar “grandiose,” transcription of two enzymes grandAand grandB increases. You have generated a series of deletion mutants (strains a-e), all of which affect transcription from this operon. You are able to measure the production of the two enzymes because they can also use chemical X as a substrate, turning chemical X pink in the presence of grandA, and blue in the presence of grandB. Presence of both enzymes will thus appear as purple. (Chemical X itself has no color.)
Color of colony when:
Mutation grandiose absent grandiose present
None (WT) white purple
a- white pink
b- white blue
c- purple purple
d- white white
e- purple purple
You create some partial diploids with F’ plasmids carrying wild type versions of genes and find the following results in the presence of chemical X. Note that genotypes vary and may include multiple genotypes:
Genotype of Color of colony when:
Chromosome: Genotype of F’: grandiose absent grandiose present
a+b+c+d+e+ a+b+c+d–e– white purple
a+b–c–d+e+ a–b+c+d+e– blue purple
a+b+c–d+e– a–b+c–d+e+ pink purple
a+b+c+d–e+ a–b+c–d+e+ pink pink
Using these data, answer the following questions:
Structural gene for grandA enzyme:
Structural gene for grandB enzyme
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