The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: enzymes are proteins.) To complete this project, it may be useful for you to review the Scientific Method Tutorial (found the Course Content section of the classroom under the Science Learning Center link) and the Scientific Method lab (Lab 1), so that you can better understand how to design an experiment. It may also be helpful for you to review your textbook and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. Feel free to refer to observations and information from Lab 4 as you complete the Final Applied Project (see the questions below). Or in other words, it is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity.
As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme and the acid, as well as explain your experimental protocol (this information will help you to answer question 2). You must also thoroughly explain how the aIDition of the acidic fluid impacted the overall reaction process (this information will help you to answer question 4).
Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.
You may need all or some of the following, depending on your experimental design:
Materials from your lab kit:
You may choose to use aIDitional materials (different acidic solutions and/or different organisms and/or different substrate(s) if you chose to look at an enzyme other than catalase).
Outline (2%) submit in assignment folder.
I suggest you include the following in your outline:
Final Applied Lab Project: write a paper that includes the following:
1. Title page: title, your name, course name, semester
2. Introduction: introduce your project; include needed background information, the question(s) that you are asking and a clear hypothesis for your experiment.
3. Design an experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis.
4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph. Remember to include the unit of measure.
5. Use your knowledge about enzymes and acids to interpret and discuss your results. It may be necessary for you to refer to your textbook and/or use other information resources. What effect does the acidic treatment have on the enzyme activity? Looking back, how could you have improved your experiment?
6. What is your conclusion? Was your hypothesis supported?
7. Cite all reference sources used (including text book) and provide a reference section with citations in APA format
Why Work with Us
Top Quality and Well-Researched Papers
We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.
Professional and Experienced Academic Writers
We have a team of professional writers with experience in academic and business writing. Many are native speakers and able to perform any task for which you need help.
Free Unlimited Revisions
If you think we missed something, send your order for a free revision. You have 10 days to submit the order for review after you have received the final document. You can do this yourself after logging into your personal account or by contacting our support.
Prompt Delivery and 100% Money-Back-Guarantee
All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.
Original & Confidential
We use several writing tools checks to ensure that all documents you receive are free from plagiarism. Our editors carefully review all quotations in the text. We also promise maximum confidentiality in all of our services.
24/7 Customer Support
Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.
Try it now!
How it works?
Follow these simple steps to get your paper done
Place your order
Fill in the order form and provide all details of your assignment.
Proceed with the payment
Choose the payment system that suits you most.
Receive the final file
Once your paper is ready, we will email it to you.
No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.
No matter what kind of academic paper you need and how urgent you need it, you are welcome to choose your academic level and the type of your paper at an affordable price. We take care of all your paper needs and give a 24/7 customer care support system.
Admission Essays & Business Writing Help
An admission essay is an essay or other written statement by a candidate, often a potential student enrolling in a college, university, or graduate school. You can be rest assurred that through our service we will write the best admission essay for you.
Our academic writers and editors make the necessary changes to your paper so that it is polished. We also format your document by correctly quoting the sources and creating reference lists in the formats APA, Harvard, MLA, Chicago / Turabian.
If you think your paper could be improved, you can request a review. In this case, your paper will be checked by the writer or assigned to an editor. You can use this option as many times as you see fit. This is free because we want you to be completely satisfied with the service offered.